Basics of homology-directed repair (HDR) using CRISPR-Cas9 Selection of CRISPR RNAs (crRNAs) for HDR Recommendations for design of a synthetic oligo or plasmid donor template Application of endogenous ...

Adoptive cell therapy based on ex-vivo expanded tumor-infiltrating lymphocytes (TILs) has historically been most effective in ...

Researchers have developed a machine learning model that predicts Cas9 proteins that can be tailored with designer properties for therapeutic use.

Plant breeding plays a vital role in ensuring global food security by increasing crop yields, improving nutritional quality ...

Genome editing has advanced at a rapid pace with promising results for treating genetic conditions—but there is always room ...

Genome editing with various CRISPR-Cas molecule complexes has ... The slow variant is called homology-directed repair. Researchers want to use this method of repair because it allows the precise ...

The precise insertion of DNA sequences using the homology-directed repair (HDR) pathway is one potential use of the CRISPR/Cas genome editing technology. Various circumstances could influence the ...

Genome editing has advanced at a rapid pace with promising results for treating genetic conditions-but there is always room for improvement.

Using gene editing with CRISPR-Cas9, the team has been able to study these proteins without altering ... Dec. 13, 2024 — Microorganisms produce a wide variety of natural products that can be ...

This linearized template, bound to Cas9-RNP, is then delivered to cells to promote precise knock-in via homology-directed repair. This method significantly enhances the precision and efficiency of ...

More information: Baolei Yuan et al, Modulation of the microhomology-mediated end joining pathway suppresses large deletions and enhances homology-directed repair following CRISPR-Cas9-induced DNA ...