Advances in the gene-editing technology known as CRISPR-Cas9 over the past 15 years have yielded important new insights into the roles that specific genes play in many diseases. But to date this ...

introduces double-strand breaks that recruit DNA repair machinery. Despite its successes, the CRISPR-Cas9 system has several limitations, including off-target cutting. Over the years, scientists have ...

However, when it comes to inserting exogenous DNA using homology-directed repair mechanisms, CRISPR editing can be woefully inefficient. Yet, inserting DNA is crucial to engineering CAR T cells.

However, DNA editing is not without its risks ... but also we created a tool,” said Nemudraia, who hopes to use the CRISPR-guided cut-and-repair system to further explore RNA repair mechanisms.

Ryotaro Hashizume and colleagues use the CRISPR-Cas9 gene editing system to ... Suppressing chromosomal DNA repair ability increased the rate of duplicate chromosome elimination.

Using CRISPR technology, Cantor and Dr. Whalen ... However, restoring double strand DNA repair functions in breast cancer cells did not save the cells from dying, thus demonstrating that these repair ...

Genome editing with various CRISPR-Cas molecule complexes ... The latter requires a copyable template for repair to accurately rejoin the DNA at the cut site. The slow variant is called homology ...

The essence of CRISPR is simple: it’s a way of finding a specific bit of DNA inside a cell. After that, the next step in CRISPR gene editing is usually to alter that piece of DNA. However ...

The precise insertion of DNA sequences using the homology-directed repair (HDR) pathway is one potential use of the CRISPR/Cas genome editing technology. Various circumstances could influence the ...

Using CRISPR technology, Cantor and Dr. Whalen ... However, restoring double-strand DNA repair functions in breast cancer cells did not save the cells from dying, thus demonstrating that these ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

Advances in the gene-editing technology known as CRISPR-Cas9 over the past 15 years have yielded important new insights into ...