In correlative light-electron microscopy (CLEM ... Green colour and black precipitate represent GFP-tagged ER-protein and blue colour chromosomes. (M. Puhka). Image 2 Epifluorescence image (inset in A ...
Due to the wave nature of light, any point source of light (even from a single GFP molecule with a size of 3 nm) is observed by the microscope as a small diffracted blur. As a result, the spatial ...
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