The stained cells were imaged using a fluorescence microscope. Alkaline phosphatase (ALP) staining The BMSCs were inoculated on slides, transfected and subjected to simulated weightlessness for 48 h.

The Shandon Sequenza Immunostaining Center can accommodate batch runs of all current methods of immunoperoxidase staining, including indirect peroxidase, avidin-biotin complex (ABC), ...

The experiment was performed after osteogenic induction for 7 and 14 days of cultured BMSCs within membranes. ALP staining was processed using an ALP staining kit (Beyotime, China). Quantification of ...