Accurately counting viable cells in a sample is crucial for various biological assays, including normalization and assessing drug impacts on cell proliferation for cancer research. Regular cell ...

Shihabeddin et al utilized single-cell RNA-Seq analysis of adult P23H zebrafish animals to identify transcription factors (e2fs, Prdm1a, Sp1) expressed selectively in neural progenitors and immature ...

Reproducibility of cell-based assays is one of the key points when it comes to reliability of experimental data. Despite of choosing the appropriate assay type, there are many more factors to be ...

One of the most familiar and widely used methods for quantifying cell proliferation is the measurement of tritiated ... N.J., offers both a [14C]-thymidine and a [3H]-thymidine uptake assay system, ...

DNA repair assay in PNKP-T118A expressing cells throughout cell cycle, and an alternative method of SSB repair assay using BrdU incorporation, as well as several control/follow-up experiments ...

Fluorescent Detection Compared to Other Methods Outside of fluorescence, colorimetry is another method of detecting target compounds or cells within a sample. In colorimetric assays, the target ...

We thus investigated whether induced pluripotent stem cell (iPSC ... (TUNEL) assay (Millipore, Burlington, MA,United States) was performed accordingly to the manufacturer’s instructions on sections of ...

Kandel et al. (5) demonstrate that MUFAs are enriched in neural stem and progenitor cells and are critical for their proliferation and survival. Blocking MUFA generation by inhibition of stearoyl-CoA ...

Methods: Cell proliferation ability was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays, 5-bromo-2-deoxyuridine (BrdU) staining and soft agar colony formation ...