Basics of homology-directed repair (HDR) using CRISPR-Cas9 Selection of CRISPR RNAs (crRNAs) for HDR Recommendations for design of a synthetic oligo or plasmid donor template Application of endogenous ...

Adoptive cell therapy based on ex-vivo expanded tumor-infiltrating lymphocytes (TILs) has historically been most effective in ...

Researchers at the University of California, Berkeley, boost the CRISPR/Cas9 system’s gene-editing success ... “We have found that Cas9-mediated HDR frequencies can be increased by rationally ...

Genome editing has advanced at a rapid pace with promising results for treating genetic conditions—but there is always room ...

CRISPR-Cas9 is effective at generating small ... However, when it comes to inserting exogenous DNA using homology-directed repair mechanisms, CRISPR editing can be woefully inefficient.

Plant breeding plays a vital role in ensuring global food security by increasing crop yields, improving nutritional quality ...

When the CRISPR Cas9 protein is added to a cell along with a piece of guide RNA, the Cas9 protein hooks up with the guide RNA and then moves along the strands of DNA until it finds and binds to a ...

Genome editing has advanced at a rapid pace with promising results for treating genetic conditions-but there is always room for improvement.

The precise insertion of DNA sequences using the homology-directed repair (HDR) pathway is one potential use of the CRISPR/Cas genome editing technology. Various circumstances could influence the ...

CRISPR/Cas9 allows for precise modifications to the genome, including knock-ins, knockouts, transcriptional activation and repression, and base conversions. This technology has been used to treat ...