2-D gel electrophoresis is the most commonly used method in biomedicine to separate even thousands of proteins in a complex sample on a single gel. Even though the technique is quite known, there is ...

For complex samples, fractionation is usually required, for example, SDS-PAGE or 2D gel electrophoresis. Protein samples will be digested with protease in gel or in solution and resulted peptides will ...

Current active projects include: (1) Development of a hybrid chip-based and automated two-dimensional electrophoresis platform for high-speed, high-throughput and sensitive protein analysis. Our ...

Two-dimensional polyacrylamide gel electrophoresis, or 2D PAGE, was introduced in 1975 concurrently by Joachim Klose 7 and Patrick H. O’Farrel. 8 In the first dimension, proteins are separated ...

For the 2D gel electrophoresis, 600 μg of the proteins was resuspended in 500 μL rehydration buffer. Subsequently, a commercially available IPG strip gel (GE Healthcare Immobiline DryStrip pH 4–7) was ...

Figure 1. One dimensional SDS polyacrylamide gel electrophoresis using the UVP GelSolo. Mouse serum was run on the gel in 1:2 dilution series from 20 μg to 39 ng on a 4 - 12 % Bolt Bis-Tris Plus ...