(C) A549 cells were transfected with a p53:MDM2 NanoBRET Protein-Protein Interaction pair and frozen for later use. Transfected cells displayed high levels of cell viability post-thaw.

Methods: Xenografts (p53 wild type [wt] A549, p53 mutant H1299 and p53 null Calu6) were generated in the leg of nude mice by i.m. injection of 1- 5 X 10 6 tumor cells grown in vitro. Tumors were ...

DO7, a p53 specific antibody, and total and phospho-p21 antibodies were optimized for multiplex detection. Bleomycin (50 mU/mL) was used to activate p53 in A549 p53 wt cells, HT29 and SkBr3 p53 mutant ...

CLSP-1025 targets the p53 R175H mutation, which is prevalent in a range of solid tumors such as colorectal, esophageal, gastric, gynecological, lung, pancreatic and prostate cancers.